Omni-C Kit

700+ Genome Assemblies

Produce the highest quality
genome assembly.

For those looking for the ultimate scaffolding tool, Dovetail Omni-C data delivers uniform, shotgun-like sequence coverage enabling genotyping and haplotype phasing, alongside long-range information characteristic of Hi-C data. Take your assembly to the next level with a fully haplotype-resolved genome.

The Omni-C kit has optimized DNase I, a sequence-independent endonuclease, to reproducibly digest chromatin, delivering all the characteristics of a Hi-C approach without the sequence bias inherent to restriction enzyme-based Hi-C approaches.

Get enriched long-range cis reads, and more complete contact matrices when viewing chromatin conformation and looping interactions. Omni-C data gives you the most complete genome-wide view for all your genome assembly, phasing, structural variant, and SNP research.

Chromosome scale haplotype assemblies of genomes with HiFi and Omni-C technologies

Haplotype-resolved assembly provides a complete picture of genomes and genetic variations. Most of the existing assembly algorithms either provide haplotype collapsed assembly or require pedigree information for phasing. New long-range sequencing approaches, such as PacBio’s HiFi technology , combined with Dovetail Omni-C libraries, a proximity ligation technology that captures chromosome-scale interactions with uniform sequencing coverage comparable to traditional shotgun sequencing libraries, make full chromosome scale haplotype resolved genome assemblies possible.

With these emerging datatypes coupled with a computational pipeline, we generated chromosome-scale phased assembly for NA12878 haplotypes with ~ 148.5 Mbp scaffold N50 for each haplotype assembly. Our assemblies phased ~ 92% of total heterozygous SNVs with more than 99% accuracy on each chromosome and showed high base-level agreement with the reference genome. Similar results were obtained for another human genome sample (HG002). Haplotype resolved assemblies for both genomes showed more than 99.99% base level accuracy with the reference genome. These results are comparable to the ones obtained with trio binning based methods such as TrioCanu.

We further applied our computational methods to sequencing data generated from Atlantic Bluefin Tuna (ABFT) genome. This genome is more heterozygous than human genome (0.01 vs 0.001). We obtained chromosome-scale assemblies for both the haplotypes with scaffold N50 of ~33.67 Mbp. Both the haplotypes when aligned to each other showed around 98.47% which is in concordance with the expected heterozygosity.

Uncover the role of SNPs, SVs, and Chromosome Phasing

Unsure how single nucleotide polymorphisms (SNPs), structural variants (SVs), or chromosome phasing impacts disease progression? Want to know if a SNP impacts a gene a megabase away? The Omni-C kit lets you dive deeper into the mechanisms of action to help you find answers. The improved coverage of Omni-C libraries enables genome-wide SNP calling, generate high-resolution data that can detect large structural variants, and deliver chromosome phasing information with low switch error rates – all with one library. Get a better understanding of the relationship between the genome and phenotype to potentially uncover the missing link for a particular disease. Elucidate mechanisms associated with SNPs that fall into non-coding regions to annotate their functional significance.

Omni-C Kit Overview

Dovetail Genomics has done all the protocol optimization and reagent qualification for you, so you can hit the ground running. The protocol eliminates the finicky sonication step used by other methods to improve reproducibility and is validated and well documented to ensure the data quality. Dovetail is dedicated to your success and has a team of support scientist ready for consultation whenever you need it.

Our Kits & Services

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Customer testimonials

  • "Our centre provides Genomic services to the scientific community over a wide range of research interests. The customer success team at Dovetail® goes above and beyond to help us through any questions or concerns we have in the course of using Dovetail® kits, whether it is for validated or non-validated sample types. This helps to ensure successful outcomes for our research community."

    Tonia Russell,
    Manager of Next Generation Sequencing - Long Read Services at Ramaciotti Centre for Genomics

  • "We are extremely happy with Dovetail’s Omni-C genome assembly of Japanese stiltgrass, now published in Genome Biology and Evolution. Invasive plant species are underrepresented among high-quality genomes, and we now have an invaluable resource for studying one of North America’s most damaging invasives. This has been a critical tool in our studies of trait variation, genomics, adaptation, and aspects of invasion biology that otherwise would have been impossible."

    Craig Barrett, West Virginia University

    https://academic.oup.com/gbe/article/13/11/evab238/6413638

  • Dovetail did an amazing job assembling the genome of annual water hyacinth (Eichhornia paniculata) with PacBio HiFi and Omni-C sequences. We got a chromosome level assembly with a very high BUSCO score (97.2%). We are using it for whole genome alignments to look for the structural variations that make up the tristyly supergenes.

    Haoran Xue, University of Toronto

  • "Based on a previously assembled genome sequence of a wild tomato relative Solanum habrochaites PI127826 using Dovetails Genomics technology helped us to obtain a chromosome-level assembly for most of our favorite wild tomato relative Solanum habrochaites Pi127826. Dovetails technology was nicely able to complement previous available 10X Genomics and Optical Mapping information yielding not only an improved assembly but also a genome annotation. Altogether, this new reference genome serves as a new foundation for our attempt to understand the basis of relevant traits into cultivated tomato."

    Marc Galland, University of Amsterdam

  • "Through the test of our several genome projects, Dovetail’s Hi-C kit has stable and excellent performance in plants, mammals and poultry. By using Dovetail Hi-C and HiRise assembly pipeline, we can obtain high-quality genomes at chromosome level. Taking eggplant as an example, we have obtained the highest quality eggplant genome so far, with contig N50=5.3 Mb and scaffold N50=93.9 Mb. In the future, we will use Dovetail’s Hi-C technology in more genome projects."

    Yongchao Niu, Biozeron Shenzhen, Inc.

  • "We have a project to sequence the genome of three Dugesia flatwom species, animals with around 2 Gbp genomes, for developing diverse studies on their evolution. The only genomes published till now for this group belong to a species from a close genus Schmidtea mediterranea for which, until very recently, only a highly fragmented genome was available. For the first two species of our project, Dovetail using PacBio and HiRise have generated a chromosome level assembly, which seems to me nearly unbelievable. I thank Dovetail for their professionality and good results. With these genomes we will be able to perform studies on chromosomal reorganizations and the effects of long-term fissiparity."

    Marta Riutort, Biodiversity Research Institute (IRBio)

  • "Highly heterozygous bivalve genomes are notoriously hard to assemble, even using long-read technologies. Dovetail’s Omni-C technology allowed us to assemble a high-quality chromosome-level genome forMytilus edulisdirectly from a draft long-read assembly. This high-quality genome will enable us to study presence-absence variation in the appears to be a key element of the adaptability of the genusMytilusand generate important genetic resources for these commercially important bivalves."

    Tiago Hori, Atlantic Aquafarms

  • "We sent Dovetail tissue samples and they sent us a high quality genome, with prompt and expert technical support, at a great price. Can’t ask for anything else and we’re planning to use their services again. At the Harris lab (www.fishbonelab.org) this genome will enable comparative genomics and population genetics analyses to unravel the ocean quahog’s secrets of exceptional longevity, up to 507 years."

    Stephen Treaster, Harvard Medical School

  • "We generated a reference-quality genome Solemya velum (Bivalvia: Protobranchia) to allow us to test hypotheses about mollusc genome evolution and the symbiotic interactions in marine molluscs. 10X Genomics, PacBio Long-reads and Hi-C data was leveraged for molluscan genome assembly. Iterations and combinations of each data type have resulted in a 2.4 GB reference genome with a scaffold N50 of 200 MB and a BUSCO completeness score of 93.7%."

    Vanessa Gonzalez, Smithsonian Institution